Tutorials

This gallery contains demonstrations of various image processing tools from the ASPIRE package, implemented using simulated data.

API Introduction

Newcomers should start here to get an overview of ASPIRE’s primitive structures and interfaces, building up to a short simulated ab-initio pipeline.

ASPIRE Configuration

ASPIRE Configuration

ASPIRE-Python Introduction

ASPIRE-Python Introduction

Ab-initio Pipeline Demonstration

Ab-initio Pipeline Demonstration

Component Examples

Selected components demonstrate some common tasks while discussing usage and configuration.

2D Covariance Estimation

2D Covariance Estimation

3D Image Orientation

3D Image Orientation

ASPIRE Image Class

ASPIRE Image Class

Apple Picker

Apple Picker

Basic Image Array

Basic Image Array

CTF: Contrast Transfer Function

CTF: Contrast Transfer Function

Class Averaging

Class Averaging

Covariance 3D Estimation

Covariance 3D Estimation

Data Downloader

Data Downloader

Generating 3D Volume Projections

Generating 3D Volume Projections

Image Expansion

Image Expansion

Image Preprocessing

Image Preprocessing

Micrograph Sources

Micrograph Sources

Relion Projection Interoperability

Relion Projection Interoperability

Starfile

Starfile

Weighted Volume Reconstruction

Weighted Volume Reconstruction